Date of Defense

Spring 3-15-2001

Department

Biological Sciences

First Advisor

Christine Byrd, Biological Sciences

Second Advisor

Silvia Rossbac, Biological Sciences

Abstract

There is a quantifiable reduction in the volume of the olfactory bulb of zebrafish following peripheral deafferentation (1). Apoptosis, programmed cell death, is evident in the bulb following deafferentation and is at peak at one hour and twenty-four hours after the surgery (5). A TNEL (TdT-mediated deoxyuridine triphosphate dUTP nick end labeling) method was used to stain cells undergoing apoptosis (3). To investigate what types of cells are dying, a tyrosine hydroxylase (TH) antibody was used to tag the cells. Both stains used fluorescence for easy comparison. One hour after removal of the olfactory organ, there was apoptosis in the nerve layer and TH in the glomerular layer. After twenty-four hours, the apoptosis increased into both layers and the TH levels decreased in the glomerular layer. Where there was overlap between the two stains, there were no double-labeled cells. Thus, the cells undergoing apoptosis after peripheral deafferentation were not TH-expressing periglomerular neurons.

Access Setting

Honors Thesis-Campus Only

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