Date of Defense
Gyula Ficsor, Biological Sciences
Leonard Ginsberg, Biological Sciences
Susan Lentz, Biological Sciences
The Suppression Subtractive Hybridization (SSH) method has previously been used to generate tissue-specific cDNA libraries and to compare gene expression in different types of tissues. We propose that the SSH method can be modified to serve as a mutation detection method. This modified SSH method would scan the entire genome for sequence disruptions in DNA. Two plasmids have been chosen to serve as a model system. One plasmid is a PGL-2 plasmid, and the second plasmid is a PGL-2 plasmid that contains a 935 base pair (bp) segment of the G6PDH gene. The goal of this project is to detect the 935 bp insert in the PGL-2 plasmid. The purpose of the plasmid model system is to demonstrate that the modified SSH method is feasible, and successful as a mutation detection method.
Fuentes, Jennifer L., "The Modification of the Suppression Subtractive Hybridization Method into a Mutation Detection Method Using a Plasmid Model System" (1998). Honors Theses. 159.
Honors Thesis-Campus Only