Date of Defense

Spring 4-13-1999

Department

Biological Sciences

First Advisor

Silvia Rossbach, Biological Sciences

Second Advisor

Alexander Enyedi, Biological Sciences

Third Advisor

Robert Eisenberg, Biological Sciences

Abstract

The author describes the use of transmission electron microscopy (TEM), scanning electron microscopy (SEM), and lipopolysaccharide (LPS) profiling to isolate and completely characterize iridescent pseudomonads found living within cattail plants. Seven different bacterial strains were isolated and analyzed using these methods. They have been identified to the genus level using all of the resulting biochemical information as well as rep-PCR and GC-FAME results. A total of over 9000 mutants were created and screened for non-display of the iridescent phenotype, and the membrane structures of wild type (Iri+) and control (Iri-) bacteria were examined using TEM to identify differences correlated to presence of iridescence.

Access Setting

Honors Thesis-Campus Only

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