Date of Defense

Spring 4-13-2007

Department

Biological Sciences

First Advisor

Wendy Ransom-Hodgkins, Biological Sciences

Second Advisor

John Geiser, Biological Sciences

Third Advisor

Pamela Hoppe, Biological Sciences

Abstract

Eukaryotic Elongation Factor One Alpha (eEF1A) is an abundant protein found in every cell of every organism and is involved in functions concerning protein synthesis and degradation, the cytoskeleton, and signaling. How these functions are regulated is not yet understood for this protein. Regulation of the protein may be controlled by transcription of the genes encoding the protein or the stability and modification of the protein. The goal of this project was to determine the stability of eEf1A proteins in Arabidopsis thaliana. Protein stability was determined by treating tissues with cycloheximide, an inhibitor of protein synthesis. Gel electrophoresis and western blot analysis was used to visualize a decrease in protein concentration and calculate the protein half-life in the cell.

Access Setting

Honors Thesis-Open Access

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