Date of Defense
4-22-2016
Date of Graduation
4-2016
Department
Chemistry
First Advisor
John Spitsbergen
Second Advisor
John Jellies
Third Advisor
John-Mary Vianney
Abstract
Glial Cell Line-Derived Neurotrophic Factor (GDNF) is a protein expressed throughout the mammalian nervous system that serves several protective functions. Originally discovered in the brain, where it supports the survival of dopaminergic neurons, GDNF has since been shown to be expressed in skeletal muscle and is vital for the maintenance of motor neurons. The relationship between muscle hypertrophy, and GDNF is not well understood. The purpose of this study was to examine the relationship between hypertrophy and GDNF by inducing hypertrophy in C2C12 skeletal muscle cells using leucine and creatine, inhibiting hypertrophy using ethanol and measuring GDNF content. As measured by enzyme linked immunoabsorbent assay, GDNF content was reduced following all treatments, therefore a clear relationship between hypertrophy and GDNF content could not be established.
Recommended Citation
Waters, Daniel, "Effects of Creatine, Leucine, and Ethanol on Glial Cell Line-Derived Neurotrophic Factor in C2C12 Skeletal Muscle Cells" (2016). Honors Theses. 2731.
https://scholarworks.wmich.edu/honors_theses/2731
Access Setting
Honors Thesis-Open Access
Included in
Biochemistry Commons, Chemistry Commons, Other Biochemistry, Biophysics, and Structural Biology Commons