Date of Defense

8-18-1993

Department

Chemistry

First Advisor

Adrian Vanderweilen, The UpJohn Company

Second Advisor

Kiyoshi Tsuji, The UpJohn Company

Third Advisor

Donald Schreiber, Chemistry

Abstract

Sodium dodecyl sulfide (SDS) non-acrylamide gel-filled capillary columns are a new technology being used for analysis and separation of biotechnology-derived proteins. This research was to compare this new technology to the current methods of SDS polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance size-exclusion chromatography (HPSEC). The molecular mass of four different recombinant proteins were determined by two commercialized SDS non-acrylamide gel-filled capillary columns, SDS-PAGE, and HPSEC. The data obtained showed that the SDS non-acrylamide gel-filled capillary columns were compatible with the SDS-PAGE technique for molecular mass determination. HPSEC was shown to be unreliable for molecular weight determination. SDS non-acrylamide gel-filled capillary columns have the advantages of being fast reliable, automated, and has improved reproducibilty and quantification.

Access Setting

Honors Thesis-Open Access