Date of Defense
8-18-1993
Department
Chemistry
First Advisor
Adrian Vanderweilen, The UpJohn Company
Second Advisor
Kiyoshi Tsuji, The UpJohn Company
Third Advisor
Donald Schreiber, Chemistry
Keywords
biotechnology, chromatography, recombinant proteins, reproduction, reproducibility, quantification
Abstract
Sodium dodecyl sulfide (SDS) non-acrylamide gel-filled capillary columns are a new technology being used for analysis and separation of biotechnology-derived proteins. This research was to compare this new technology to the current methods of SDS polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance size-exclusion chromatography (HPSEC). The molecular mass of four different recombinant proteins were determined by two commercialized SDS non-acrylamide gel-filled capillary columns, SDS-PAGE, and HPSEC. The data obtained showed that the SDS non-acrylamide gel-filled capillary columns were compatible with the SDS-PAGE technique for molecular mass determination. HPSEC was shown to be unreliable for molecular weight determination. SDS non-acrylamide gel-filled capillary columns have the advantages of being fast reliable, automated, and has improved reproducibilty and quantification.
Recommended Citation
Andres, DeVon, "SDS Non-Acrylamide Polymeric Gel-Filled Capillary Electrophoresis for Molecular Size Separation of Protein" (1993). Honors Theses. 632.
https://scholarworks.wmich.edu/honors_theses/632
Access Setting
Honors Thesis-Open Access
Included in
Biochemistry Commons, Biotechnology Commons, Inorganic Chemicals Commons, Medical Biochemistry Commons, Medicinal-Pharmaceutical Chemistry Commons, Pharmaceutical Preparations Commons