Date of Award

12-1991

Degree Name

Master of Arts

Department

Chemistry

First Advisor

Dr. Susan Stapleton

Second Advisor

Dr. Michael E. McCarville

Third Advisor

Dr. Donald Berndt

Fourth Advisor

Dr. Leonard Ginsberg

Access Setting

Masters Thesis-Open Access

Abstract

In chick embryo hepatocytes in culture, triiodothyronine (T3) stimulates enzyme activity, mRNA level and transcription rate for both fatty acid synthase (FAS) and malic enzyme (ME). Recent evidence has demonstrated the effects of selenate, molybdate and chromate on various physiological processes. Little information, however, is available on the effects of these metal ions on the T3- regulated gene expression for FAS and ME.

In chick embryo hepatocytes incubated in a chemically defined medium, addition of sodium selenate (20 μM) coincident with T3 almost completely inhibited the T3-induced stimulation of FAS and ME activity and accumulation of their respective mRNA's. Neither ammonium molybdate nor ammonium chromate has any effect on either the enzyme activity or mRNA level of FAS or ME under similar experimental conditions.

Included in

Biochemistry Commons

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