Date of Award
12-2011
Degree Name
Master of Science
Department
Chemical and Paper Engineering
First Advisor
Dr. Dewei Qi
Second Advisor
Dr. Dan Fleming
Third Advisor
Dr. Alexandra Pekarovicova
Fourth Advisor
Dr. Jan Pekarovic
Access Setting
Masters Thesis-Campus Only
Abstract
The focus ofthis thesis is to investigate the effects of enzymatic hydrolysis on recycled paperboard mill sludge. The sludge is landfilled at 45 %wt solids at a cost to the manufacturer. Hydrolyzing the cellulose present in the sludge would allow for a reduction of sludge and provide a product in D-glucose that could be used as an intermediate product in the production of ethanol, levulinic acid, gluconic acid, sorbitol, or polylactic acid. This research examined the composition of the sludge and the rate and yield of sugar production during enzymatic hydrolysis of recycled paperboard mill sludge under various conditions. The research investigated the effects of washing/de-ashing of sludge, cellulase activity, and cellulase enzyme complex.
The experiments were conducted at 50°C, pH of 4.8-5.0, using Accellerase 1500 and Cellic CTec2 cellulase enzymes at 10 and 40 FPU/g-cellulose for 24 hours. The results of the experiments showed that the sludge possessed 47 % wt. carbohydrates available for enzymatic hydrolysis. It was found that washing of sludge greatly influenced the yield of reducing sugar, while enzyme activity affected the rate of enzymatic hydrolysis. The highest rate and yield were obtained by washed sludge at 40 FPU/g-celluose using the Cellic CTec2 enzyme complex.
Recommended Citation
Ostroski, Mark, "Investigation of the Production of Sugafrom Recylced Paperboard Mill Sludge by Enzymatic Hydrolysis" (2011). Masters Theses. 464.
https://scholarworks.wmich.edu/masters_theses/464