Date of Award

8-1993

Degree Name

Master of Science

Department

Biological Sciences

First Advisor

Dr. Leonard Beuving

Second Advisor

Dr. Robert Eversole

Third Advisor

Dr. Roger Ulrich

Fourth Advisor

Dr. William Jackson

Access Setting

Masters Thesis-Open Access

Abstract

1-Nitronaphthalene (1-NN) is an environmental contaminant that has been shown to cause pulmonary toxicity in rodents. To examine the contribution of cytochrome P-450 to the metabolism and subsequent toxicity of 1-NN, rats were pretreated with chloramphenicol, a suicide substrate of P-450. To determine if inflammation plays a role in 1-NN toxicity, rats were pretreated with cyclophosphamide to deplete neutrophils. In both experiments, morphological analysis of the lower airways was performed with light and electron microscopy. A second group of animals was examined for biochemical indicators of inflammation and cell damage. The parameters examined were cell content, protein content and lactate dehydrogenase activity in bronchoalveolar fluid, and tissue myeloperoxidase activity. The results showed that pretreatment with chloramphenicol prevented the cytotoxicity associated with 1-NN, but not the inflammatory response. Pretreatment with cyclophosphamide did not prevent 1-NN cytotoxicity, but did prevent neutrophildependent aspects of inflammation.

Included in

Cell Biology Commons

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