Date of Defense
Our early concept regarding the genetic composition of individuals was that of essential monomorphism. We believed individuals were genetically homozygous for most genes and heterozygous at only very few loci. Thus, the phenotypic variability expressed among individuals was the result of variability at relatively few loci interacting with a multitude of environmental parameters while interpopulation differences were the result of differential survival and reporudction of individuals within these populations. The concept of monomorphism was shattered in 1965 when electrophoretic methods, combined with histo-chemical staining techniques, revealed an unexpectedly large amount of variation within and among diploid individuals and populations (Lewontin and Hubby, 1966). This has made possible the identification of different enzymes with catalyze the same basic reaction. Multiple molecular forms of a single enzyme are termed isozymes. The application of electrophoretic methods to tissue extracts and body fluids revealed a large amount of isozymic variation.
Baxter, Terry L., "Isozyme Variation in Bacillus Cereus Var. Mycoides From Diverse Natural Origins" (1975). Honors Theses. 130.
Honors Thesis-Campus Only