Date of Defense
Carbohydrate metabolism investigation has long been, and is an interesting, involved, and fruitful field of research. Now that the basics of major carbohydrate pathways have been elucidated, researchers have turned to the specifics and fine points: genetics, enzyme stereochemistry, and others. Bacteria have long been of primary importance as laboratory tools in carbohydrate pathway analysis. The bacteriologist can observe several bacterial generations in a matter of hours. The important microbial catabolic pathways are the Embden-Meyeroff pathway, referred to as glycolysis, the hexose monophosphate shunt, and the Entner-Doudoroff pathway. Among other things, bacterial cells require two things: an energy source to provide energy for the work done by the organism, and a carbon source from which the cell can build and replace various cellular structures. Glycolysis serves as an energy generating pathway by which glucose and other carbon compounds similar to glucose which can be converted into glycolytic intermediates are degraded to pyruvate with the resultant production of the high energy compound adenosine triphosphate (ATP). If Pseudomonas aeruginosa glucose oxidase mutants can be obtained, a systematic investation of glucose dissimilation can be explored.
Fagerburg, Rodger, "Induction of Entner-Doudoroff Enzymes in Pseudomonas Aeruginosa" (1972). Honors Theses. 158.
Honors Thesis-Campus Only