Date of Defense
Wendy Ransom-Hodgkins, Biological Sciences
John Geiser, Biological Sciences
Pamela Hoppe, Biological Sciences
Eukaryotic Elongation Factor One Alpha (eEF1A) is an abundant protein found in every cell of every organism and is involved in functions concerning protein synthesis and degradation, the cytoskeleton, and signaling. How these functions are regulated is not yet understood for this protein. Regulation of the protein may be controlled by transcription of the genes encoding the protein or the stability and modification of the protein. The goal of this project was to determine the stability of eEf1A proteins in Arabidopsis thaliana. Protein stability was determined by treating tissues with cycloheximide, an inhibitor of protein synthesis. Gel electrophoresis and western blot analysis was used to visualize a decrease in protein concentration and calculate the protein half-life in the cell.
Watry, Jennifer, "Stability of Eukaryotic Elongation Factor One Alpha Protein in the Cell" (2007). Honors Theses. 194.
Honors Thesis-Open Access