Date of Defense

Summer 8-1980


Biological Sciences

First Advisor

Robert C. Eisenberg

Second Advisor

M.E. McCarville

Third Advisor

J.L. Foote


Mannitol binding protein (MBP) from Pseudomonas aeruginosa, strain PAO, was extracted and purified although not to homogeneity. Maximum stability of the protein activity at 4°C was attained using a citrate-phosphate buffer at pH 5.0. The approximate molecular weight was confirmed to be ca 36,000 daltons. Evidence for the periplasmic character of MBP was also obtained by adapting a spheroplast coldshock procedure originally developed for preparing membrane vesicles from P. aeruginosa. A comparison of the relative distribution of MBP to glucose-6-phosphate dehydrogenase in periplasmic, cytoplasmic and membrane fractions established that MBP is a periplasmic constituent of P. aeruginosa.

Access Setting

Honors Thesis-Campus Only