Date of Defense
Charles Ide, BIological Sciences
Anna Jelaso, Environmental Studies
Alexander Enyedi, Biological Sciences
Polychlorinated biphenyls, or PCBs, are ubiquitous environmental contaminants with a wide range of deleterious human and ecological health effects. PCBs had a wide range of industrial applications due to their stability and resistance to degradation. The manufacture of PCBs was banned in the early 1970s in the United States, but levels of PCGs in the environment still persist. This study attempted to use in situ hybridization (ISH) technology to locate and quantify expression of three developmental genes in Xenopus laevis after exposure to the commercial PCB mixture Aroclor 1254. A parent study to this paper used microarray and real time PCR technology to identify gross gene expression in X. laevis after similar exposures to Aroclor 1254. Although the authors were unable to localize gene expression, the primary goal of the study to optimize ISH for use for X. laevis was met.
Olsen, Bjorn T., "In Situ Hybridization on the African Clawed Frog Xenopus Laevis After Exposure to Aroclor 1254" (2002). Honors Theses. 229.
Honors Thesis-Campus Only