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This paper analyzes and optimizes efficiency in the yeast transformation protocol using electroporation. Three variables were tested for optimization, they are the following: DNA concentration, cell density and electroporation voltage. Further verification of transformation was done through the analyzation of HPLC data to ensure protein production. The results conclude that DNA concentration was the most important dependent variable for transformation efficiency. A voltage of 2000V is the optimal condition to achieve efficient yeast transformation. Cell density had no observational difference in transformation efficiency at the range of DNA concentration necessary for research use. Improving this efficiency of the yeast transformations helps to stream-line the discovery phase of new product development.
Ruell, Lilly, "High-Efficiency Transformation of Yeast by Electroporation" (2020). Honors Theses. 3320.
Honors Thesis-Open Access