Date of Defense




First Advisor

Adrian Vanderweilen, The UpJohn Company

Second Advisor

Kiyoshi Tsuji, The UpJohn Company

Third Advisor

Donald Schreiber, Chemistry


biotechnology, chromatography, recombinant proteins, reproduction, reproducibility, quantification


Sodium dodecyl sulfide (SDS) non-acrylamide gel-filled capillary columns are a new technology being used for analysis and separation of biotechnology-derived proteins. This research was to compare this new technology to the current methods of SDS polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance size-exclusion chromatography (HPSEC). The molecular mass of four different recombinant proteins were determined by two commercialized SDS non-acrylamide gel-filled capillary columns, SDS-PAGE, and HPSEC. The data obtained showed that the SDS non-acrylamide gel-filled capillary columns were compatible with the SDS-PAGE technique for molecular mass determination. HPSEC was shown to be unreliable for molecular weight determination. SDS non-acrylamide gel-filled capillary columns have the advantages of being fast reliable, automated, and has improved reproducibilty and quantification.

Access Setting

Honors Thesis-Open Access