Date of Defense
Adrian Vanderweilen, The UpJohn Company
Kiyoshi Tsuji, The UpJohn Company
Donald Schreiber, Chemistry
biotechnology, chromatography, recombinant proteins, reproduction, reproducibility, quantification
Sodium dodecyl sulfide (SDS) non-acrylamide gel-filled capillary columns are a new technology being used for analysis and separation of biotechnology-derived proteins. This research was to compare this new technology to the current methods of SDS polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance size-exclusion chromatography (HPSEC). The molecular mass of four different recombinant proteins were determined by two commercialized SDS non-acrylamide gel-filled capillary columns, SDS-PAGE, and HPSEC. The data obtained showed that the SDS non-acrylamide gel-filled capillary columns were compatible with the SDS-PAGE technique for molecular mass determination. HPSEC was shown to be unreliable for molecular weight determination. SDS non-acrylamide gel-filled capillary columns have the advantages of being fast reliable, automated, and has improved reproducibilty and quantification.
Andres, DeVon, "SDS Non-Acrylamide Polymeric Gel-Filled Capillary Electrophoresis for Molecular Size Separation of Protein" (1993). Honors Theses. 632.
Honors Thesis-Open Access