Date of Defense




First Advisor

Susan Stapleton, Chemistry

Second Advisor

Leonard Beuving, Biomedical Sciences

Third Advisor

Lonnie Adams, The Upjohn Company


pioglitizone treatment, hemoglobin, haptoglobin, plasma proteins


ABSTRACT This project investigated plasma proteins showing a concentration correlation with pioglitizone treatment in KKAy and C57 mice, as identified from SDS-PAGE gels. The proteins were isolated using chromatographic and electrophoretic techniques. They were then identified through sequencing and 2-dimensional SDS-PAGE analysis as hemoglobin and haptoglobin. Currently unpublished data on the sequence of mouse haptoglobin was determined. Several studies were also performed to determine the significance of the trends originally seen in the preliminary study. It was determined that there is a significant decrease in hemoglobin levels in both KKAy and C57 mice in response to pioglitizone treatment. There is also a significantly higher level of hemoglobin present in the plasma of KKAy mice than in the plasma of C57 mice. The trends for the haptoglobin levels were found to be insignificant.

Access Setting

Honors Thesis-Campus Only