Date of Defense
Paul Bombardt, Pharmacia & Upjohn
Susan Burns, Chemistry
A possible screening model was developed for the determination of highly bound drugs using high performance liquid chromatography and a human serum albumin column. Retention times generated with the chromatography were used to place the compounds in one of two categories, either greater than 90.0% bound or less than 90.0% bound. The retention time of Quinine HC1 was used as a separation point for these categories as it has been determined by previously developed methods to have a binding value of 90.0%. Values obtained were then compared with published values of the binding for the compounds tested. Of the twenty three compounds tested, only six appeared in categories conflicting with the published values.
Vallad, Allison J., "A Possible Screening Model for Determining Highly Bound Drugs Using High Performance Liquid Chromatography and a Human Serum Albumin Column" (1998). Honors Theses. 730.
Honors Thesis-Campus Only