Date of Award
Master of Science
Masters Thesis-Open Access
Total mouse satellite DNA was isolated in cesium chloride gradients in the presence of the dye Hoechst 33258. This satellite fraction was digested with Bst Nl, an isoschizomer of the restriction enzyme Eco RII, and the 234 base pair monomer fragment was cloned into the plasmid vector pBS. Dideoxynucleotide sequencing of the cloned DNA yielded significant homology with the published consensus sequence of mouse satellite DNA (92%).
Boyle, Timothy Patrick, "Cloning and Sequencing of the Eco RII Monomer Fragment of Satellite DNA from the Mouse Mus Musculus" (1989). Masters Theses. 1109.