Author

Baumgarten

Date of Award

8-1986

Degree Name

Master of Science

Department

Biological Sciences

First Advisor

Dr. Robert C. Eisenberg

Access Setting

Masters Thesis-Open Access

Abstract

Studies on the purification of a mannitol binding protein (MBP) from Pseudomonas aeruginosa PAO were performed. Utilizing the alkaline isoelectric point (pi 8.3) of MBP, pH gradients were used to elute MBP from carboxymethylcellulose cation exchange resins. Purification of MBP was monitored by sodium dodecyl sulfate-polyacrylam ide gel electrophoresis (SDS-PAGE). Only one protein band was seen on SDS-PAGE from MBP fractions elutedat pH 7-8 and 8 .2. Although pH gradients appear to give good purification from cation exchange resins, these procedures lead to loss of MBP activity.

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