Expression of Hla-Dr Is Up-Regulated in Microglia/Macrophages of the Multiple System Atrophy


Junjie Hu

Date of Award


Degree Name

Master of Science


Biological Sciences

First Advisor

Dr. Charles F. Ide

Second Advisor

Dr. Cindy Linn

Third Advisor

Dr. Robert R. Eversole


HLA-DR, microglia, up-regulated, MSA, ICC

Access Setting

Masters Thesis-Abstract Only


Multiple system atrophy (MSA) is a progressive neurodegenerative disorder (Quinn, 1989). Activation ofCD68+ microglia correlates with system degeneration in MSA, suggesting a possible inflammatory reaction in MSA pathogenesis (Ishizawa et al., 2004). HLA-DR, a major histocompatibility complex class II molecule, has been found in Parkinson's disease and multiple sclerosis (McGeer et al., 1988). However, the role of HLA-DR in MSA has not been described. To examine a possible role of HLA-DR in MSA, we conduct an immunocytochemistry (ICC) study on MSA and control cerebellar sections. HLA-DR distribution is quantitatively measured using image analysis on cerebellar sections immunostatned with anti-HLA-DR and anti-CD68 antibodies. ANOVA analysis is performed to delineate differences between control and MSA groups. Results indicate near significant differences between control and MSA tissue for both HLA-DR and CD68 in all foliar areas analyzed. Thus, HLA-DR-positive microglia and macrophages may be involved in inflammatory interactions in MSA. In other neurodegenerative disease such as Multiple Sclerosis, this combination of immune markers underlies a T-cell attack on myelinated axons. This mav be the case in MSA, since cerebellar white matter tracts are demyelinated.


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