Introduction: Gallbladder duplication is a rare congenital anomaly. Preoperative diagnosis is essential for prevention of intra operative complications. Based on our review of the literature, this is the first description of gallbladder duplication treated with single-incision laparoscopic surgery (SILS) in a pediatric patient. Case Presentation: A 14 year-old girl presented to the pediatric surgery clinic with a 4-month history of right upper quadrant abdominal pain, nausea, and low- grade fevers. Preoperative imaging revealed gallbladder duplication. The single incision laparoscopic technique was employed. Intra operative ndings included two gallbladders that appeared to share a common wall. There were two parallel cystic ducts that inserted separately into the common hepatic duct. Gross pathology revealed two adjacent gallbladders separated by a thin septum, each with its own cystic duct. The postoperative course was unremarkable, and the patient was without symptoms at the 1 month follow-up visit. Conclusion: Gallbladder duplication is a rare congenital anomaly. This case report demonstrates successful treatment by excision using the single incision laparoscopic approach.