Date of Award
8-1993
Degree Name
Master of Science
Department
Biological Sciences
First Advisor
Dr. Leonard J. Beuving
Second Advisor
Dr. Robert Eversole
Third Advisor
Dr. Roger Ulrich
Fourth Advisor
Dr. William Jackson
Access Setting
Masters Thesis-Open Access
Abstract
1-Nitronaphthalene (1-NN) is an environmental contaminant that has been shown to cause pulmonary toxicity in rodents. To examine the contribution of cytochrome P-450 to the metabolism and subsequent toxicity of 1-NN, rats were pretreated with chloramphenicol, a suicide substrate of P-450. To determine if inflammation plays a role in 1-NN toxicity, rats were pretreated with cyclophosphamide to deplete neutrophils. In both experiments, morphological analysis of the lower airways was performed with light and electron microscopy. A second group of animals was examined for biochemical indicators of inflammation and cell damage. The parameters examined were cell content, protein content and lactate dehydrogenase activity in bronchoalveolar fluid, and tissue myeloperoxidase activity. The results showed that pretreatment with chloramphenicol prevented the cytotoxicity associated with 1-NN, but not the inflammatory response. Pretreatment with cyclophosphamide did not prevent 1-NN cytotoxicity, but did prevent neutrophildependent aspects of inflammation.
Recommended Citation
Green, Steven Michael, "Inhibition of Cytochrome P-450 but Not Neutrophil Depletion Prevents the Pulmonary Toxicity Associated with 1-Nitronaphthalene" (1993). Masters Theses. 784.
https://scholarworks.wmich.edu/masters_theses/784