The Neurobehavioral Consequences of Gestational and Chronic Atrazine Exposure in Male and Female Sprague Dawley Rats
Date of Award
Doctor of Philosophy
Dr. Lisa E. Baker
Dr. Cynthia Pietras
Dr. Alan Poling
Dr. Charles Ide
Atrazine, behavior, toxicology, motor coordination, dopamine, striatum
The adverse health risks of exposure to the herbicide, atrazine, in humans are not fully understood. Although numerous studies have demonstrated atrazine to be an endocrine disrupter, the neurobehavioral consequences of atrazine exposure have not been thoroughly examined. The aim of this study was to investigate the effects of environmentally-relevant levels
of gestational followed by continued chronic atrazine exposure on motor function, learning and memory, anxiety, and striatal dopamine content in rodents. Pregnant Sprague Dawley rats were treated by gavage with 100 µg/kg atrazine (ATZ low), 10 me/kg atrazine (ATZ high), or vehicle on
gestational day l through postnatal day (PND) 21 (weaning). Upon weaning, offspring continued daily vehicle or atrazine gavage treatments for an additional six months. Rats were subjected to a series of behavioral assays, including locomotor activity assessments, a walking beam task, an operant spatial discrimination reversal task, a Morris Water Maze task, and a light-dark box test. Twenty-four hours after the last treatment, animals were euthanized, and tissue samples from the striatum, nucleus accumbens, and median eminence were analyzed for dopamine (DA) and 3,4-dihydroxyphenylacetic acid (DOPAC). Results revealed ATZ low males
displayed increased locomotor activity at 1 month of age, while ATZ high males displayed decreased locomotor activity at 6 and 7 months of age. Both ATZ low and ATZ high males and females had impairments in walking beam performance on PND 37-46 and at 22 weeks of age. No differences were observed in learning and memory while ATZ high males had increased anxiety-like behavior at 7 months of age. Neurochemical assays revealed decreased striatal DA and DOPAC in ATZ high males and females and in ATZ low males. ATZ low females had decreased striatal Dn and increased DA turnover. These results suggest environmentally relevant exposures to atrazine have the potential to disrupt brain regions and behaviors associated with motor functioning. Further investigation of the potential impact of atrazine exposure on neurological development in humans and increased risk for Parkinson disease may be warranted.
Walters, Jennifer L., "The Neurobehavioral Consequences of Gestational and Chronic Atrazine Exposure in Male and Female Sprague Dawley Rats" (2014). Dissertations. 393.