Date of Defense
Mary J. Ruwart, Upjohn Company
Leonard Ginsberg, Biological Sciences
Stephen Friendmann, Biological Sciences
This in situ study was conducted with the intent to resolve the discrepancy found in Rop et al.'s (1992) rat intestinal perfusion study. Rop et al.'s (1992) results showed a substantial lumenal peptide uptake, but reported an almost insignificant amount of peptide found in the mesenteric vein collection sample. This disappearance-reappearance differential was found to be an artifact, and was rectified. Following intestinal perfusion results showed no peptide absorption was occurring with compounds U-88272 and U-88937. The introduction of verapramil to block the multi-drug resistance pump, P-glycoprotein, and facilitate peptide uptake again resulted in no intestinal absorption of U-88272. These results do not correspond with those predicted by heptane-ethylene glycol partition coefficients and Caco-2 permeabilities (Conradi, et al., 1991). Further studies must be conducted to determine the cause for poor peptide absorption, and why results did not concur with the heptane-ethylene glycol and Caco-2 predictions in this specific case.
Bushouse, Wendy A., "Peptide Absorption in a Rat Intestinal Perfusion Model" (1992). Honors Theses. 144.
Honors Thesis-Campus Only