Date of Defense

8-14-2014

Date of Graduation

8-2014

Department

Biological Sciences

First Advisor

Charles Ide

Second Advisor

John Spitsbergen

Abstract

Adults Stem cells are multipotent cells, which can divide mitotically and transform into more than one cell type such as retinal cell, cardiac cell, neural cells. I examined the retinal development and regeneration in Xenopus laevis, tadpoles (stage 46) and compared them with the normal Xenopus laevis, tadpoles. Several steps were applied to do this research such as surgical step for creating nasal 1/3 eye fragment on one animal and dorsal 1/3 eye fragment on the other animal), healing and fixation, paraffin embedding and sectioning, immunohistochemistry using SOX 2 and BLBP markers as the primary antibodies, and image analysis. This research showed that the surgical eye (nasal 1/3) appeared having less neural stem cells than the control eye. In addition, the sox 2 marker apparently shared similar expressions with BLBP marker because the BLBP marker showed overlapping stained with the sox-­‐2 marker.

Access Setting

Honors Thesis-Restricted

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