Date of Defense

Spring 4-10-1977

Department

Biological Sciences

First Advisor

Leonard Beuving

Abstract

The purpose of this study was to determine the likelihood of a role of aryl hydrocarbon [benzo(a)pryene] hydroxylase (AHH) activity in 7,12-deimethylbenz(a)anthracene (DMBA) induced carcinogensis. To test this hypothesis, drugs known to be inducers and inhibitors of AHH were administered concurrently with DMBA. The following treatment groups were established: oil-control, chrysene-control, DMBA, DMBA+benzoflavone (BF), an inhibitor, DMBA+pyrene, an inducer, and DMBA+chrysene, an inducer. Latency period, growth rate, and incidence of tumors was observed. THe abdominal-inguinal glands were removed and observed for hyperplastic alveolar nodules, hyperplastic areas, and small tumors. It was shown that the inducers of AHH activity, pyrene and chrysene, decreased tumor incidence and increased latency period as compared to controls. The group treated with BF, an inhibitor of AHH, was similar to controls. The incidence of hyperplastic alveolar nodules, growth rate and regression due to overiectomy were the same for all groups. This imples that pyrene and chrysene affect only the initiation phase of DMBA-induced carcinogensis.

Access Setting

Honors Thesis-Campus Only

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