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Cell-free protein synthesis (CFPS) is a method used to make proteins without the use of a living cell, while utilizing extracted essential cellular transcription and translational machinery from the living cell. In this study, two in-house cell-free extraction protocols were compared and the synthesis GFP was compared to a commercial CFPS kit. Overall, this study aims to analyze how usable and reproducible both CFPS protocols are for undergraduate students. Even with some optimization of sonication and T7 RNA Polymerase supplementation, neither protocol resulted in detectable GFP expression.
Laurin, Andrea, "E. coli-based Cell-Free Protein Synthesis Protocol Comparison and Optimization" (2021). Honors Theses. 3467.
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