Date of Defense

8-16-2021

Date of Graduation

8-2021

Department

Biological Sciences

First Advisor

David Huffman

Second Advisor

Alexandra Haase

Abstract

Cell-free protein synthesis (CFPS) is a method used to make proteins without the use of a living cell, while utilizing extracted essential cellular transcription and translational machinery from the living cell. In this study, two in-house cell-free extraction protocols were compared and the synthesis GFP was compared to a commercial CFPS kit. Overall, this study aims to analyze how usable and reproducible both CFPS protocols are for undergraduate students. Even with some optimization of sonication and T7 RNA Polymerase supplementation, neither protocol resulted in detectable GFP expression.

Access Setting

Honors Thesis-Restricted

Restricted to Campus until

10-12-2022

Available for download on Wednesday, October 12, 2022

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