Date of Award


Degree Name

Master of Science


Biological Sciences

First Advisor

Dr. Leonard C. Ginsberg

Access Setting

Masters Thesis-Open Access


Interleukin-1 (IL-1) demonstrated a concentration dependent, specific binding to the human, large granular lymphocyte cell line, YT.NCI with a dissociation constant of 0.2 nM. There were approximately 500 IL-1 receptors/cell.

IL-1 binding to YT.NCI cells could be inhibited by unlabeled-IL-1 and the plant lectins, WGA and Con A. Lectin inhibition of IL-1 binding could be completely abrogated by exposing lectins to specific simple sugars prior to the addition of ligand.

Tunicamycin, an inhibitor of glycosylation, decreased IL-l binding to YT cells in a reversible, time- and concentration-dependent manner.

These observations suggest that glycosylation of cell surface proteins may be important for IL-1 receptor orientation, expression, and/or functional ligand binding. Furthermore, the demonstration that specific plant lectins effectively compete with iodinated IL-1 for IL-1 receptor occupancy could facilitate the establishment of conditions for successful isolation and purification of IL-1 membrane receptors via affinity chromatography on immobilized lectins.

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Biology Commons