Date of Award
Master of Science
Dr. L. Beuving
Dr. G. J. Kolaja
Dr. L. C. Ginsberg
Masters Thesis-Open Access
The objective of this research was to evaluate receptor localization and distribution on normal and diabetic cells by electron microscopic analysis.
To achieve this objective, gold-adsorbed and ferritin-conjugated macromolecules were used to determine the location and concentration of specific receptor binding sites on the cells surfaces. To facilitate the use of these ultra-structural histocytochemical markers, normal and diabetic Chinese hamster and other nondiabetic cells were either grown in vitro on ultra thin plastic films or embedded in Lowicryl K4M and pre- or post-labelled with these marker molecules.
Analysis of the data obtained from electron micrographs of these labelled cells was used to determine the densities and distributions of the various diabetic-related markers on the cells surfaces.
Kincaid, "Receptor Localization and Distribution on Normal and Diabetic Cells by Electron Microscopy" (1988). Master's Theses. 1198.