Author

Kincaid

Date of Award

12-1988

Degree Name

Master of Science

Department

Biological Sciences

First Advisor

Dr. L. Beuving

Second Advisor

Dr. G. J. Kolaja

Third Advisor

Dr. L. C. Ginsberg

Access Setting

Masters Thesis-Open Access

Abstract

The objective of this research was to evaluate receptor localization and distribution on normal and diabetic cells by electron microscopic analysis.

To achieve this objective, gold-adsorbed and ferritin-conjugated macromolecules were used to determine the location and concentration of specific receptor binding sites on the cells surfaces. To facilitate the use of these ultra-structural histocytochemical markers, normal and diabetic Chinese hamster and other nondiabetic cells were either grown in vitro on ultra thin plastic films or embedded in Lowicryl K4M and pre- or post-labelled with these marker molecules.

Analysis of the data obtained from electron micrographs of these labelled cells was used to determine the densities and distributions of the various diabetic-related markers on the cells surfaces.

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Biology Commons

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