Date of Award


Degree Name

Master of Science


Biological Sciences

First Advisor

Dr. L. Beuving

Second Advisor

Dr. G. J. Kolaja

Third Advisor

Dr. L. C. Ginsberg

Access Setting

Masters Thesis-Open Access


The objective of this research was to evaluate receptor localization and distribution on normal and diabetic cells by electron microscopic analysis.

To achieve this objective, gold-adsorbed and ferritin-conjugated macromolecules were used to determine the location and concentration of specific receptor binding sites on the cells surfaces. To facilitate the use of these ultra-structural histocytochemical markers, normal and diabetic Chinese hamster and other nondiabetic cells were either grown in vitro on ultra thin plastic films or embedded in Lowicryl K4M and pre- or post-labelled with these marker molecules.

Analysis of the data obtained from electron micrographs of these labelled cells was used to determine the densities and distributions of the various diabetic-related markers on the cells surfaces.

Included in

Biology Commons