Date of Award


Degree Name

Master of Arts



First Advisor

Dr. Jochanan Stenesh

Second Advisor

Dr. Michael McCarville

Third Advisor

Dr. Dale Warren

Access Setting

Masters Thesis-Open Access


14C-leucine was incorporated into th e proteins of the mesophile Bacillus licheniformis and the thermophile Bacillus stearothermophilus. Aliquots of the cell suspension were treated with chloramine-T in order to decarboxylate the 14C-leucine that was released as the proteins degraded. The 14CO2 was trapped and counted. This allowed for the determination of the extent of intracellular protein degradation at various tem peratures and at various stages of the growth cun/e. For the mesophile, the extent of degradation increased with temperature from 30° C to 55° C. For the thermophile, no significant amount of degradation occurred until about 30 hours had passed, at which time the extent of degradation increased for the next 24 hours. The results show that slow turnover may be an important characteristic of thermophily.

Included in

Biochemistry Commons