A Study of the Mannitol Binding Protein Active Transport System and a Catabolite Repression Resistant Mutant in Pseudomonas Aeruginosa
Date of Award
Master of Science
Dr. Robert C. Eisenberg
Dr. M. E. McCarville
Dr. S. B. Friedman
Masters Thesis-Open Access
Cold-shock extraction procedures were used for the efficient release of mannitol binding protein. Magnesium chloride and cold-shock released 92% of the total whole cell mannitol binding protein activity without release of detectable cytoplasmic marker protein glucose-6- phosphate dehydrogenase. Arsenate inhibited mannitol uptake to the same extent as azide. The simultaneous loss and gain of mannitol binding protein activity with mannitol uptake activity in mutants and reventants respectively, was consistant with periplasmic mannitol binding protein as a required component of mannitol uptake. A catabolite repression resistant mutant for mannitol uptake was isolated and described. This mutant, designated WEP-1, escaped catabolite repression of mannitol uptake in the presence of succinate. Downstream inducible enzymes of carbohydrate catabolism did not escape succinate repression to the same extent as did the early proteins required for mannitol uptake. This appears to be the first catabolite repression resistant mutant described for Pseudomonas.
Wolff, Joseph Arthur, "A Study of the Mannitol Binding Protein Active Transport System and a Catabolite Repression Resistant Mutant in Pseudomonas Aeruginosa" (1984). Masters Theses. 1576.