Date of Award
Master of Science
Dr. Karim Essani
Dr. Robert Eversole
Dr. Bruce Bejcek
Generation, tanapoxvirus, ankyrin, deletion, mutant
Masters Thesis-Open Access
The host range of poxviruses is highly regulated and is limited to certain hosts. A number of genes in different poxviruses have been identified that contribute to cell tropism. The ankyrin (ANK) repeat genes encode for proteins characterized by the repetitive motifs in its amino acid sequence. These genes have been found to play a role in determining the ability of poxviruses to replicate in certain cell types. Tanapoxvirus (TPV) Kenya genome analysis indicated the presence of six ANK repeat genes of which T146R, T147R and T148R are present in the terminal part of the TPV-Kenya genome. To understand the possible role of T146R in determining the cell tropism, an insertional knockout TPV (TPV-ΔT146R) was constructed. The ability of TPV-ΔT146R to replicate in a variety of human colorectal cancer cell lines and human malignant melanoma cells was also tested. Cell monolayers were observed for viral cytopathic effect at 96 hours post infection (h.p.i.) indicated that both TPV-Kenya and TPV-ΔT146R replicated in most of the human colorectal cancer cell lines. Taken together, these results provide evidence that the open reading frame T146R in TPV is a non-essential gene.
Suryawanshi, Yogesh, "Generation of Tanapoxvirus Ankyrin Repeat Gene Deletion Mutant for Evaluating its Role in Cell Tropism" (2012). Masters Theses. 34.