Author

Harris

Date of Award

6-2004

Degree Name

Master of Science

Department

Chemistry

First Advisor

Dr. Subra Muralidharan

Second Advisor

Dr. Donald R. Schreiber

Third Advisor

Dr. Susan Stapleton

Access Setting

Masters Thesis-Open Access

Abstract

Protein recognition by HPLC, based on histidine residues, was achieved by incorporating a copper chelating ligand covalently bonded to a polystyrene/divinyl benzene substrate, into column packing. The metal ion was bonded to the substrate in two ways: randomly, where the copper was bound to the ligand after the substrate polymerization and imprinted, where the copper was bound to the ligand before the substrate polymerization. The effect of this templating was measured by protein separation parameters of the resulting HPLC chromatographs. The hypothesis of this thesis is the imprinted substrate will have a higher copper content, and with coppers affinity toward histidine, will have better separation capabilities than the randomly prepared substrate.

Included in

Chemistry Commons

Share

COinS