Date of Award
6-2004
Degree Name
Master of Science
Department
Chemistry
First Advisor
Dr. Subra Muralidharan
Second Advisor
Dr. Donald R. Schreiber
Third Advisor
Dr. Susan Stapleton
Access Setting
Masters Thesis-Open Access
Abstract
Protein recognition by HPLC, based on histidine residues, was achieved by incorporating a copper chelating ligand covalently bonded to a polystyrene/divinyl benzene substrate, into column packing. The metal ion was bonded to the substrate in two ways: randomly, where the copper was bound to the ligand after the substrate polymerization and imprinted, where the copper was bound to the ligand before the substrate polymerization. The effect of this templating was measured by protein separation parameters of the resulting HPLC chromatographs. The hypothesis of this thesis is the imprinted substrate will have a higher copper content, and with coppers affinity toward histidine, will have better separation capabilities than the randomly prepared substrate.
Recommended Citation
Harris, Len A., "Metal Ion Imprinted Polymers for Biomolecular Recognition" (2004). Masters Theses. 4332.
https://scholarworks.wmich.edu/masters_theses/4332