Date of Award
Master of Science
Dr. Charles Spilman
Dr. George Gerritsen
Dr. Leonard Ginsberg
Masters Thesis-Open Access
The binding characteristics of glucocorticoid receptors in the liver cytosol of Chinese hamsters of the BE, BD (diabetic), and AV (nondiabetic) lines were studied and compared using a charcoal adsorption technique and 3H-dexamethasone. High affinity receptors(Kd ≃ 3 x 10-9 M) were found with concentrations of 180 fmoles/mg protein in the nondiabetic and 260 fmoles/mg protein in the diabetic hamsters. Behavior of the receptor-steroid complex in 5 to 20% sucrose gradients of low and high salt concentrations (0.0 and 0.3 M KCl) and the competitive binding of other steroids were consistent with findings for rat liver glucocorticoid receptors.
Affinity, specificity and homogeneity under assay conditions were similar for glucocorticoid receptors of diabetic and nondiabetic hamsters. The 44% greater receptor concentration in diabetic hamsters was not statistically (p > 0.05) different from that in nondiabetic hamsters, but a trend was indicated. The importance of this trend relative to the diabetic state has not yet been determined.
Gibson, Richard E., "Glucocorticoid Receptors in Liver Cytosol of Adrenalectomized Diabetic and Nondiabetic Chinese Hamsters" (1980). Masters Theses. 4508.