Date of Award
4-1995
Degree Name
Master of Science
Department
Biological Sciences
First Advisor
Dr. Robert Eisenberg
Second Advisor
Dr. Susan Stapleton
Third Advisor
Dr. Karim Essani
Access Setting
Masters Thesis-Open Access
Abstract
An assay based on the spectrophotometeric properties of NAD/NADH was developed to detect the degradation of L-arabinose to L-arabinolactone. This assay was used to determine the expression of the L-arabinose utilization genes contained in Pseudomonas fluorescens and in the plasmid, designated pPZ221, contained in Pseudomonas putida. The results from this assay, in conjunction with various growth kinetics, indicate that pPZ221 is inducible and repressible for L-arabinose utilization. Thus, the P. fluorescens chromosomal insert in pPZ221 for L-arabinose utilization does appear to contain the regulatory genes necessary for controlling the expression of an L-arabinose operon.
Recommended Citation
Wierenga, Carol M., "Characterization and Expression of Pseudomonas Fluorescens L-Arabinose Dehydrogenase: Genetic Elements in Pseudomonas Putida" (1995). Masters Theses. 4527.
https://scholarworks.wmich.edu/masters_theses/4527