Author

Wierenga

Date of Award

4-1995

Degree Name

Master of Science

Department

Biological Sciences

First Advisor

Dr. Robert Eisenberg

Second Advisor

Dr. Susan Stapleton

Third Advisor

Dr. Karim Essani

Access Setting

Masters Thesis-Open Access

Abstract

An assay based on the spectrophotometeric properties of NAD/NADH was developed to detect the degradation of L-arabinose to L-arabinolactone. This assay was used to determine the expression of the L-arabinose utilization genes contained in Pseudomonas fluorescens and in the plasmid, designated pPZ221, contained in Pseudomonas putida. The results from this assay, in conjunction with various growth kinetics, indicate that pPZ221 is inducible and repressible for L-arabinose utilization. Thus, the P. fluorescens chromosomal insert in pPZ221 for L-arabinose utilization does appear to contain the regulatory genes necessary for controlling the expression of an L-arabinose operon.

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Biology Commons

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