Author

Neering

Date of Award

12-1993

Degree Name

Master of Science

Department

Biological Sciences

First Advisor

Dr. Karim Essani

Second Advisor

Dr. Susan Stapleton

Third Advisor

Dr. Robert Eisenberg

Access Setting

Masters Thesis-Open Access

Abstract

Tanapox virus (TPV), of the genus Yatapoxvirus, is a human poxvirus that was originally isolated from the Tana River Valley in Kenya in 1962. TPV produces a mild disease characterized by a short febrile illness associated with one or more nodular skin lesions. Although there have been limited studies on TPV, other poxviruses such as vaccinia virus, have been extensively characterized and their genomic DNAs sequenced. In order to examine the molecular features of TPV, we have cloned approximately 17% of the genomic DNA. TPV DNA was digested with Pstl restriction endonuclease and cloned into pUC19. The Pstl-L fragment, composed of 2,108 base pairs, has been sequenced. Computer analyses show there are several potential open reading frames (ORF) within this fragment. The largest ORF consists of 239 amino acids (27 kDa). None of the ORFs analyzed show a high degree of homology with other poxviruses.

Included in

Biology Commons

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