Date of Award
12-1993
Degree Name
Master of Science
Department
Biological Sciences
First Advisor
Dr. Karim Essani
Second Advisor
Dr. Susan Stapleton
Third Advisor
Dr. Robert Eisenberg
Access Setting
Masters Thesis-Open Access
Abstract
Tanapox virus (TPV), of the genus Yatapoxvirus, is a human poxvirus that was originally isolated from the Tana River Valley in Kenya in 1962. TPV produces a mild disease characterized by a short febrile illness associated with one or more nodular skin lesions. Although there have been limited studies on TPV, other poxviruses such as vaccinia virus, have been extensively characterized and their genomic DNAs sequenced. In order to examine the molecular features of TPV, we have cloned approximately 17% of the genomic DNA. TPV DNA was digested with Pstl restriction endonuclease and cloned into pUC19. The Pstl-L fragment, composed of 2,108 base pairs, has been sequenced. Computer analyses show there are several potential open reading frames (ORF) within this fragment. The largest ORF consists of 239 amino acids (27 kDa). None of the ORFs analyzed show a high degree of homology with other poxviruses.
Recommended Citation
Neering, Sarah J., "Complete Nucleotide Sequence of PstI-L fragment of Tanapox Virus Genomic DNA" (1993). Masters Theses. 4535.
https://scholarworks.wmich.edu/masters_theses/4535