Date of Award
Master of Arts
Dr. Imy V. Holt
Dr. Jack Wood
Dr. Jean Lawrence
Masters Thesis-Open Access
The purpose of this investigation was to differentiate Staphylococcus aureus phage type 42D from Staphylococcus epidermidis.
The investigation involved four approaches to this differentiation. The first was a selective screening technique on specific media to point out physical and biochemical characteristics and to demonstrate the presence of specific enzymes. The second was a drug survey which was used to show basic susceptibility and resistance to ten different antibiotics. The third was ultra-violet irradiation which was used to discern susceptibility and resistance to the soft rays of this spectrum segment. The fourth was a chromatographic procedure using labeled carbon which demonstrated certain amino acids that were present and/or synthesized by these microorganisms, and showed carbon-14 incorporation by these microorganisms.
The differentiation therefore was based on the finding of similarities and differences. Since variation sometimes points to a genetic difference, the investigation also proposed to indicate some basic genetic similarities and differences between these two strains of staphylococci.
Hosksema, "A Comparative Approach into the Differentiation of Two Strains of Staphylococci" (1966). Master's Theses. 4541.