Date of Award


Degree Name

Master of Arts


Biological Sciences

First Advisor

Dr. Imy V. Holt

Second Advisor

Dr. Jack Wood

Third Advisor

Dr. Jean Lawrence

Access Setting

Masters Thesis-Open Access



The purpose of this investigation was to differentiate Staphylococcus aureus phage type 42D from Staphylococcus epidermidis.

The investigation involved four approaches to this differentiation. The first was a selective screening technique on specific media to point out physical and biochemical characteristics and to demonstrate the presence of specific enzymes. The second was a drug survey which was used to show basic susceptibility and resistance to ten different antibiotics. The third was ultra-violet irradiation which was used to discern susceptibility and resistance to the soft rays of this spectrum segment. The fourth was a chromatographic procedure using labeled carbon which demonstrated certain amino acids that were present and/or synthesized by these microorganisms, and showed carbon-14 incorporation by these microorganisms.

The differentiation therefore was based on the finding of similarities and differences. Since variation sometimes points to a genetic difference, the investigation also proposed to indicate some basic genetic similarities and differences between these two strains of staphylococci.

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