Author

Thekkat

Date of Award

4-2007

Degree Name

Master of Science

Department

Chemistry

First Advisor

Dr. Susan R. Stapleton

Second Advisor

Dr. David S. Reinhold

Third Advisor

Dr. Sherine Obare

Access Setting

Masters Thesis-Open Access

Abstract

In liver, insulin and glucose regulate the expression of important genes for lipid and carbohydrate homeostasis. Studies on the effects of glucose and insulin, including effects on cell signaling, are vital since deficiencies in glucose utilization in cells and/or insulin secretion contribute to conditions like obesity and diabetes. In liver, the pentose phosphate pathway is one of the primary pathways that can utilize the excess carbohydrate. Glucose-6-phosphate dehydrogenase (G6PDH) is an important rate limiting enzyme in this pathway. Our laboratory has shown that both insulin and glucose induce the expression of G6PDH, although the mechanism by which this transcriptional regulation occurs is still not clear.

The transcriptional regulation by glucose on enzymes involved in carbohydrate and fatty acid metabolism like glucokinase and fatty acid synthase involves transcription factors that bind to specific sequences in the promoter regions within these genes. Building on our previous findings and on the wealth of information reported for other lipogenic genes, the aim of the present study is to determine the identity of transcription factors that are utilized in the carbohydrate regulation of G6PDH gene expression in liver cells in culture.

Included in

Chemistry Commons

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