Date of Award
Master of Science
Electrical and Computer Engineering
Dr. Massood Z. Atashbar
Dr. Bruce Bejcek
Dr. John Gesink
Masters Thesis-Open Access
Quartz Crystal Microbalances (QCMs) have been widely used for detection of various chemical and biological species in liquid media. In this work an improved binding of Protein A and IgG molecules on QCM biosensors by modifying the gold surface of the quartz crystal with an ultra thin polystyrene film followed by an acidic treatment has been reported. Protein A and IgG immobilizations on quartz crystals with the polystyrene film represented a 65% increase and a 40% increase respectively when compared to immobilization done directly onto the crystals. Atomic Force Microscopy (AFM) has been used to investigate and study the biomolecular coverage on different substrates. The results obtained using QCM and AFM are analyzed and correlated together. Simulation and modeling of the acoustic wave sensors is done using ANSYS 6.1 to enhance the understanding of the sensing phenomena and the design parameters of the sensor.
Vijh, Aditya A., "Acoustic Wave Based Biosensor" (2004). Masters Theses. 4557.