Author

Franz

Date of Award

12-2004

Degree Name

Master of Science

Department

Biological Sciences

First Advisor

Dr. Karim Essani

Second Advisor

Dr. John Geiser

Third Advisor

Dr. Brian Tripp

Access Setting

Masters Thesis-Open Access

Abstract

The initial symptoms of a Tanapoxvirus (TPV) infection are indistinguishable from smallpox, which is caused by variola virus (VV). In the post-9/11 area, reagents that can quickly differentiate a TPV infection from smallpox are very desirable, but currently unavailable. Monoclonal antibodies (mAbs) directed specifically against TPV can be used for this purpose. Six mAbs were developed and characterized that specifically bind to TPV infected owl monkey kidney (OMK) cells, but not uninfected cells. The apparent molecular mass of the TPV protein(s) recognized by 5 mAbs were calculated using the Western blot technique. One mAb demonstrated no binding using the Western blot technique, but demonstrated specific binding to TPV protein(s) by ELISA and indirect immunofluorescence microscopy. Since TPV is included in the genus Yatapoxvirus and the VV is included in the genus Orthopoxvirus, with no immunological cross-reactivity, it is presumed that these antibodies will react with TPV proteins and not VV proteins.

Included in

Biology Commons

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