Author

Haller

Date of Award

8-2001

Degree Name

Master of Science

Department

Biological Sciences

First Advisor

Dr. Karim Essani

Second Advisor

Dr. Bruce Bejcek

Third Advisor

Dr. Rob Eversole

Access Setting

Masters Thesis-Open Access

Abstract

The hybridoma technique has been used for the production of monoclonal antibodies (mAb) directed against yaba-like disease virus (YLDV). Spleen cells, obtained from female BALB/c mice immunized with YLDV, were fused with P3x63.Ag8.653 myeloma cells and hybrids were selected. Hybridoma clones were screened for production of anti-YLDV antibodies by Indirect Immunofluorescence (IF) microscopy. Positive hybridoma clones were recloned via limiting dilution and characterized. A single clone (H12) has been selected that specifically reacted with YLDV infected cells and not uninfected cells. Immunodiffusion technique determined H12 monoclonal antibody as an IgG1 isoptype immunoglobulin. H12 antibodies recognized a late YLDV protein, and cross-reacted with tanapox virus (TPV) infected cells providing additional evidence to classify YLDV and TPV as different strains of the same virus. The approximate molecular weight of the antigen has yet to be confirmed. The H12 mAb should prove invaluable in the elucidation of the virus-host interactions with YLDV and TPV potential diagnosis of these infections in humans and monkeys.

Included in

Biology Commons

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