Date of Award


Degree Name

Master of Science


Biological Sciences

First Advisor

Dr. Christine Byrd-Jacobs

Second Advisor

Dr. John Spitsbergen

Third Advisor

Dr. Karim Essani


Zebrafish, microglia, deafferentation, olfactory bulb, phagocytosis

Access Setting

Masters Thesis-Abstract Only

Restricted to Campus until



The major goal of this study was to investigate a microglial response following deafferentation in the adult zebrafish, Danio rerio, olfactory bulb. Microglia were identified in the olfactory bulb using the 4c4 antibody and phagocytic-dependent labeling (DiA). Microglia responded to both methods of deafferentation namely, permanent (cautery) and temporary (Triton X- 100). On one and three days following both permanent and temporary deafferentation there was a significant increase in microglia in the treated olfactory bulb. At five and seven days after permanent and temporary deafferentation there was no longer a significant difference between the treated and untreated olfactory bulbs, indicating that a microglia response occured following permanent and temporary deafferentation. This study has established the foundation for future microglia studies in the degeneration and reinnervation processes in the adult zebrafish olfactory bulb.