Date of Award


Degree Name

Master of Arts



First Advisor

Dr. Donald Schreiber

Second Advisor

Dr. James Howell

Third Advisor

Dr. Michael Dziewatkoski

Fourth Advisor

Dr. Tracie Sheehan

Access Setting

Masters Thesis-Open Access


A HPLC method to quantitate the mycotoxins Fumonisin B1 (FB1), Ochratoxin A(OA), and Zearalenone (ZON) was developed and validated. Mycotoxins are secondary metabolites of fungi like Fusarium, Asperigillus and Penicillium that contaminate crops. They have a wide array of chemical structures which facilitate their analytical detection.

The analytical method consists of reverse phase chromatography and fluorescence detection. Sample cleanup was performed using SAX and C18 cartridges prior to analysis on the HPLC.

The method developed for FB1 is unique in that it is more sensitive, accurate, and reproducible than the existing methods. Automation is one of the salient features that improves sensitivity of the method and. overcomes the limitations due to the instability of the FB1-OPA derivative. Simultaneous analysis of OA and ZON was made possible by varying the HPLC conditions. Extraction optimization and statistical validation studies were completed for both the methods.