Date of Award
Master of Science
Dr. Susan Stapleton
Dr. David Reinhold
Dr. Bruce Bejcek
Masters Thesis-Open Access
Chronically high blood glucose levels lead to many problems, such as insulin resistance, the hallmark of Type II diabetes. Increased flux through the hexosamine biosynthesis pathway is one mechanism by which high glucose as well as glucosamine has been shown to induce insulin resistance. This study tests the effects of glucosamine induced insulin resistance on insulin regulation of the metabolic genes glucose-6-phosphate dehydrogenase (G6PDH) and fatty acid synthase (FAS) as well as insulin responsive proteins tribbles homolog (TRIB3) and sterol regulatory element binding protein (SERBP-1c) 1c.
Selenium, a micronutrient has been shown to be an effective insulin mimetic in Type I diabetics, however its effectiveness has not been tested under Type II diabetic conditions. Thus we also assessed the ability of Se to act as an insulin mimetic agent under glucosamine induced insulin resistance.
In summary, our results show that glucosamine effects the insulin induction of the genes tested. The effect of Se, however was variable and is suggestive of different mechanisms by which it modulates gene expression.
Ruff, David L. II, "Expression of Insulin Responsive Genes in Insulin Resistant Conditions, and the Effect of Selenium on Gene Expression" (2015). Master's Theses. 670.