Application of Electrospray Ionization Mass Spectrometry for Ionic Liquids and their Biodegradation Products
Date of Award
Master of Science
Dr. Andre R. Venter
Dr. Michael J. Barcelonaq
Dr. Blair Szymczyna
Electrospray, ionization, mass spectrometry, ionic liquids, biodegradation products
Masters Thesis-Open Access
Ionic liquids (ILs) are organic salts with unique physico-chemical properties and are used as green alternatives to volatile organic solvents. ESI-MS is a sensitive technique to detect these stable, permanent ions. My research focused on (1) Identification of biodegradation products of ionic liquids and (2) Multiplexed tandem MS by modulation of ionization efficiency.
Studies, have demonstrated that ILs are toxic to the environment, which makes their biodegradability an important consideration. In this work, metabolites generated during the biodegradation of 1-butyl-3-methylimidazolium chloride (BMIM), a degradation resistant and commonly used ionic liquid, are identified using high performance liquid chromatography-mass spectrometry (LC-MS).
Multiplexed tandem MS is a method to increase sample throughput for identification of complex mixtures. However, previous multiplexed tandem MS methods mostly require expensive mass spectrometers. In this study, we demonstrate a novel method of multiplexing MS where correlation of the fragments to their respective precursors is achieved by modulating the ionization efficiencies of analytes by changing the solvent composition. This method has the potential to do powerful MS/MS experiments on inexpensive single stage mass analyzers.
Rahbarirad, Sepideh, "Application of Electrospray Ionization Mass Spectrometry for Ionic Liquids and their Biodegradation Products" (2016). Masters Theses. 691.