Date of Award


Degree Name

Master of Science



First Advisor

Dr. Susan R. Stapleton

Access Setting

Masters Thesis-Open Access


Fatty acids are utilized for energy storage in the form of triglycerides or neutral fats. One way to regulate fatty acid biosynthesis is to regulate the activity of fatty acid synthase (FAS), the enzyme that catalyzes the reaction. Previous studies have shown that regulation of FAS activity and synthesis is regulated by the hormone triiodothyronine, T3, at the level of transcription. Thus, isolation and characterization of the promoter region o f the FAS gene will enable the identification of the thyroid hormone response elements (TREs). This study could shed light on the mechanisms on how this gene can be controlled.

In this study we were able to: (a) isolate a putative FAS promoter region from chicken liver using known goose liver FAS promoter sequence and the polymerase chain reaction; (b) isolate, clone, and sequence the exon 3 of the chicken hypoxanthine phosphoribosyl transferase, HPRT, gene as a control from the experiments conducted in a; and (c) create deletions of a cloned 1.6 kb promoter region of goose fiver FAS to determine the location of the T3 response element present.