Date of Award
Doctor of Philosophy
Dr. Jochanan Stenesh
Dr. Lin Foote
Dr. Michael McCarville
Dr. Susan Stapleton
The rates of intracellular degradation of ribosomal proteins were compared in a mesophilic and a thermophilic species of Bacillus. Cells were grown under various incubation conditions in the presence of a 14^C-protein hydrolysate m order to label the ribosomes. Rates of ribosome degradation were evaluated in terms of changes in the specific activity of in vivo ribosomes as a function of incubation time. The results indicated that, under most conditions, ribosomes were degraded (in vivo) at similar rates in both the mesophile and the thermophile. In some studies, ribosome degradation rates were slightly faster for the thermophile than for the mesophile.
In limited in virro investigations, the thermostability of isolated, purified ribosomes was compared. The results showed a slightly higher stability for thermophilic ribosomes under the conditions studied.
Davis, Joe William, "Intracellular Degradation of Ribosomes in a Mesophilic and a Thermophilic Species of Bacillus" (1990). Dissertations. 2072.