Date of Award

12-1990

Degree Name

Doctor of Philosophy

Department

Chemistry

First Advisor

Dr. Jochanan Stenesh

Second Advisor

Dr. Lin Foote

Third Advisor

Dr. Michael McCarville

Fourth Advisor

Dr. Susan Stapleton

Abstract

The rates of intracellular degradation of ribosomal proteins were compared in a mesophilic and a thermophilic species of Bacillus. Cells were grown under various incubation conditions in the presence of a 14^C-protein hydrolysate m order to label the ribosomes. Rates of ribosome degradation were evaluated in terms of changes in the specific activity of in vivo ribosomes as a function of incubation time. The results indicated that, under most conditions, ribosomes were degraded (in vivo) at similar rates in both the mesophile and the thermophile. In some studies, ribosome degradation rates were slightly faster for the thermophile than for the mesophile.

In limited in virro investigations, the thermostability of isolated, purified ribosomes was compared. The results showed a slightly higher stability for thermophilic ribosomes under the conditions studied.

Comments

Fifth Advisor: Dr. Stephen Friedman

Sixth Advisor: Dr. James Howell

Access Setting

Dissertation-Open Access

Download

Included in

Chemistry Commons

Share

COinS