The Effects of Selenate, Molybdate and Chromate on the Triiodothyronine Induced Enzyme Activity and mRNA Level for Both Fatty Acid Synthase and Malic Enzyme
Date of Award
Master of Arts
Dr. Susan R. Stapleton
Dr. Michael E. McCarville
Dr. Donald Berndt
Dr. Leonard Ginsberg
Masters Thesis-Open Access
In chick embryo hepatocytes in culture, triiodothyronine (T3) stimulates enzyme activity, mRNA level and transcription rate for both fatty acid synthase (FAS) and malic enzyme (ME). Recent evidence has demonstrated the effects of selenate, molybdate and chromate on various physiological processes. Little information, however, is available on the effects of these metal ions on the T3- regulated gene expression for FAS and ME.
In chick embryo hepatocytes incubated in a chemically defined medium, addition of sodium selenate (20 μM) coincident with T3 almost completely inhibited the T3-induced stimulation of FAS and ME activity and accumulation of their respective mRNA's. Neither ammonium molybdate nor ammonium chromate has any effect on either the enzyme activity or mRNA level of FAS or ME under similar experimental conditions.
Zhu, Yuan, "The Effects of Selenate, Molybdate and Chromate on the Triiodothyronine Induced Enzyme Activity and mRNA Level for Both Fatty Acid Synthase and Malic Enzyme" (1991). Masters Theses. 1001.